Next-Generation Sequencing (NGS) Library Construction

Enabling higher throughput while increasing efficiency, and reducing hands-on time, Biomek NGS Workstations provide flexibility to maximize scheduling your day. Demonstrated methods can be run start to finish with full walk away capability, and include optional start and stop points based on the kit vendor’s recommendations.

Built using the Biomek 4000, i5 and i7 liquid handlers, Biomek NGS Workstations offer scalable automation solutions for NGS sample prep workflows including all steps involved from extraction through library construction. Pre-defined configurations for the i-Series include capability for on-deck incubation, magnetic bead separation, and shaking as well as optional enclosures and on-deck thermocycling.

Other configurations are available. Request a quote or contact us for more information.

Illumina Nextera XT DNA Library Prep Kit workflow on the i7 NGS Workstation

Illumina Nextera XT DNA Library Prep Kit workflow on the i7 NGS Workstation

Features

Biomek Method Launcher is a secure interface for method implementation without affecting method integrity, and allows users to remotely monitor the progress of the run. The manual control options provide the opportunity to interact with the instrument, if needed. Guide Labware Setup (GLS) provides the user specific text and graphical setup instructions such as reagent volume calculations and step-by-step instructions to prepare reagents and place labware for NGS library construction.

Biomek i7 Hybrid NGS Workstation Features

300 µL or 1200 µL Multichannel head with 1-300 µL and 5-1200 µL pipetting capability
Span-8 pod with fixed and disposable tips
Enhanced Selective Tip pipetting to transfer custom array of samples
Independent 360˚ rotating gripper with offset fingers optimize access to high density decks
High deck capacity with 45 positions
Orbital Shakers, peltiers, span-8 and 96 channel Tip washing for controlling sample processing
On-deck thermocycler option
Enclosure option

Biomek i5 NGS Workstation Features

Span-8 pod with fixed and disposable tips
Multichannel Enhanced Selective Tip pipetting to transfer custom array of samples
Independent 360˚ rotating gripper with offset fingers optimize access to high density decks
High deck capacity with 25 positions
Orbital Shakers, peltiers, and span-8 Tip washing for controlling sample processing
On-deck thermocycler option
Enclosure option

Biomek 4000 NGS Workstation Features

With both single-channel and multichannel pipette tools Biomek 4000 has a dynamic pipetting range of 1-1000 µL
Single Tip pipetting to transfer custom arrays of samples
Enhanced Gripper tool optimizes access to both on-deck and off-deck integrated solutions
Orbital shakers, peltiers and thermal cycling for controlling sample processing
Enclosure option

Specifications

Minimum Table Support Requirements	116.00\|63.00\|cm / 45.67\|24.80\|in
Pod and Tool Type	1μL - 300 μL 96-Channel head; 5 μL -1200 μL 96-Channel Head; 0.5 μL - 60 μL Multichannel-384 Head; Span-8 Pod

Operating System	Windows® 10
Software	SAMI EX scheduling software, Biomek PowerPack software, Biomek Method Launcher, DART 2.0 software, Biomek software
Display	22" controller screen
Operating Modes	Semi-automated, manual, controlled by operator
Autoclavable	No
Item Specifications Referenced	C23339

Resources

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Title	Description
Automated Sample Preparation Methods {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Explore our demonstrated methods for automating NGS sample prep as well as automated nucleic acid sample prep methods
201901_interview
BeckmanConnect Installation and Activation Instructions {B14306BD-BB46-4B27-BF3E-B11862085BC7}	A step-by-step guide to help you successfully install BeckmanConnect.
Highly Reproducible Automated Proteomics Sample Preparation on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Automation can relieve the burden of sample preparation while also reducing the variability between users and across experiments
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
A Highly Consistent Lowry Method on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The quantification is based on peptide bonds of proteins reacting with copper under alkaline conditions followed by copper-catalyzed oxidation of aromatic amino acids, producing blue color
High Throughput Screening {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	For drug discovery and biomarker research, screening campaigns are employed to identify potential new treatments for diseases and to answer questions that remain unknown in the scientific community.
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Beckman Coulter AMPure XP kit is a highly efficient PCR purification system that delivers superior quality DNA with no salt carryover
Transfection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The introduction of exogenous nucleic acids into cells is an essential means of studying cell biology.
A highly consistent BCA assay on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The BCA (bicinchoninic acid) assay is a popular method used in quantification of proteins
A highly consistent Bradford assay on Biomek i-Series {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Bradford protein assay is a simple, fast and sensitive method that is used to measure the concentration of total protein in a sample
Biomek Automated NGS Solutions Accelerate Genomic Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq Exome Library Prep kit is a workflow to generate indexed paired-end libraries and is specially designed to work with Formalin Fixed Paraffin Embedded (FFPE) samples
Automating Cell Line Development for Biologics {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Cell line development is a highly-involved, multi-week workflow that involves the plating, screening, and expansion of single cell-derived clones to establish a protein-expression cell line
Lab Automation Training Programs {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Real-world training options for maximizing the value of your Biomek liquid handling system.
Protection for your Workflows in Automation {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Maintenance service plan for Biomek liquid handlers
BeckmanConnect Security Highlights {749D68FB-4893-4B2D-BD6C-C662F0520F64}	This document highlights the privacy and security features in the BeckmanConnect remote management solution.
【カタログ】自動分注ワークステーションBiomek i-Series（Biomek i5 / Biomek i7） {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Biomek i-Seriesは、これまでのわれわれベックマン・コールターの30年以上のラボラトリーオートメーションシステムの取り扱いの中で皆様からのご要望から誕生しました。ユーザーが使いやすく、信頼性の高い結果を生むことができるシステム、それがBiomek i-Seriesです。
Designed for your Evolving Workflow Priorities Biomek i-Series Automated Workstations {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Your input and our experience led to the engineering of a liquid handling platform that enables you to get the trusted, reliable results you need from your application workflows
Accelerate Drug Discovery and Basic Research {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	You need a comprehensive solution, provided by a company that believes in listening as you describe your challenges, then taking a consultative approach to helping you address them.
Cell Line Development – Hit Picking {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note we discuss how hit picking was automated as part of a cell line development workflow
Cell Line Development – Limiting Dilution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note we describe the automation of limiting dilution on a Biomek i7 liquid handling workstation that overcomes a number of challenges associated with this workflow
Cell Line Development – Selection and Enrichment {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The quest to find cells that stably express and secrete antibodies or other protein products from a transfected pool of cells requires the screening of thousands of wells/cells
Automating Cell Line Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features automating cell line development with a Biomek i7 liquid handling workstation and integrated devices in order to overcome challenges for this workflow
Automating Cell Line Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell line development is the process by which the cellular machinery is co-opted to manufacture therapeutic biologics or other proteins of interest
Cell Line Development – Data Handling {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell line development is one of many workflows that has significant data challenges
Flexible ELISA automation with the Biomek i5 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Enzyme-linked immunosorbent assays (ELISAs) are a common analytical tool for detecting the presence of a protein or other molecules of interest
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Extraction of high quality DNA from formalin fixed paraffin embedded (FFPE) tissue is essential
Fully-Automated Cellular Analysis by Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Direct integration of CytoFLEX Flow Cytometer to Biomek i-Series Automated Workstations enables complete automation of sample processing and data acquisition
Continuous Cell Culture {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Continuous cell culture provides scientists the ability to readily have assay-ready plates available to test potential agents or to have large quantities of multiple cell lines available for therapies research.
FormaPure DNA Extraction kit for FFPE Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The FormaPure DNA extraction kit provides a convenient extraction process utilizing Beckman Coulter’s proprietary solid phase reversible immobilization (SPRI) chemistry to extract DNA from FFPE curls.
Illumina TruSeq Stranded mRNA Sample Preparation Kit Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Illumina TruSeq® Stranded mRNA Sample Preparation Kit protocol converts mRNA from total RNA into a template sample of known origin
Cell-Based Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell Culture is an essential workflow that enables studies to be performed in drug discovery and scientific research
Cell Line Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As biologics research continues to evolve and gain prominence in therapy development for disease states, the need for reliable and robust cell lines will greatly increase
Cell Culture Automating Cell Maintenance {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell culture is an essential workflow that enables studies to be performed in drug discovery and scientific
Cell Line Development – Limiting Dilution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Limiting dilution is a traditional approach to achieve monoclonality
Illumina TruSeq Nano DNA Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Illumina TruSeq Nano DNA Library Prep Kit enables preparation of uniquely indexed DNA libraries for single end and paired end sequencing
Consistent Cell Maintenance and Plating through Automation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell culture is a valuable yet time-consuming aspect of basic biological research and drug discovery
Simple, Yet Effective – Automating Bradford Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Excellent consistency of automated standard dilution and Bradford assay preparation
Flexible ELISA Automation with the Biomek i5 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Automated ELISA assay workflow \| Optimized liquid transfers eliminate the need for a plate washer \| High-capacity deck accommodates fresh tip usage
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAdvance Tissue Kit uses Beckman Coulter’s patented Agencourt SPRI paramagnetic bead-based technology to isolate total RNA (microRNA and mRNA).
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
Efficient Factorial Optimization of Transfection Conditions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Design of Experiment (DOE) approach can be quickly automated on the Biomek i7 Automated Workstation to identify optimal transfection conditions for a variety
Illumina TruSeq Exome Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq Exome Library Prep kit is a workflow to generate indexed paired-end libraries and is specially designed to work with Formalin Fixed Paraffin Embedded (FFPE) samples
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sample quality is of utmost importance to genomic researchers to better ensure good results when running laborious and expensive processes such as Next Generation Sequencing.
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
Automated library preparation for the MCI Advantage Cancer Panel at Miami Cancer Institute utilizing the Beckman Coulter Biomek i5 Span-8 NGS Workstation {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Here we describe the automation of the MCI Advantage Cancer Panel at Miami Cancer Institute, a 170 gene panel assay based upon the Illumina TruSight® Tumor 170 Panel, on the Beckman Coulter Biomek i5 Span-8 NGS Workstation
Accelerate Biomarker Discovery and Improve Results Using Automation {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Author: Zach Smith, MS \| Published 2017
Automating Genomic DNA Extraction from Whole Blood and Serum with GenFind V3 on the Biomek i7 Hybrid Genomic Workstation {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Here, we demonstrate a walk-away automated solution for GenFind V3 kit to purify up to 400 µL of whole blood using the Beckman Coulter Biomek i7 Hybrid Genomics Workstation.
Efficient Factorial Optimization of Transfection Conditions Poster {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Automate the creation of 96 factorial combinations of different transfection reagents, concentrations, and cell numbers to improve transfection efficiency
Fully-Automated Cellular Analysis by Flow Cytometry Poster {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	The Biomek i7 Automated Workstation was used to automate the complete cellular workflow for induction and analysis of apoptosis in two cancer lines
Manufacturing Processes for Engineered T-Cell Therapy – CAR-T {749D68FB-4893-4B2D-BD6C-C662F0520F64}	Brochure presenting the CAR-T cell therapy workflow and associated product portfolio
Manufacturing Processes for Engineered T-Cell Therapy – CAR-T	Brochure presenting the CAR-T cell therapy workflow and associated product portfolio
Cell Line Development – Hit Picking {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The basic concept of hit picking is simple – 1) identify hits based on assay data and 2) consolidate these hits into a new labware in an identifiable way
Efficient Factorial Optimization of Transfection Conditions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nucleic acid transfection is a procedure used in nearly any cellular laboratory and the abundance of commercially available reagents has made this a seemingly simple endeavor
Automated NGS Library Construction & Nucleic Acid Sample Prep Methods {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Demonstrated Methods on Biomek Automated Workstations are developed for sample preparation kits following a vendor’s published manual protocol

Next-Generation Sequencing (NGS) Library Construction

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