Isolamento do Sangue (RNA)

Construído sobre tecnologia com base em esferas paramagnéticas de imobilização reversível em fase sólida (Solid Phase Reversible Immobilization, SPRI), o sistema Agencourt RNAdvance Blood é um processo de isolamento de ácido ribonucleico (RNA). Permite que a comunidade de ciências da vida purifique RNA de alta qualidade de sangue coletado com os tubos PAXgene*. O kit é formatado para processar uma placa de 96 poços em cerca de 3,5 horas.

Choose a From Blood Model

RNAdvance Blood Workflow Steps

Genomics RNAdvance Blood Workflow

Product Features

Overview
RNAdvance Blood isolates RNA from various RNA preservation tubes (e.g. PAXgene Blood RNA Tubes).

High Quality RNA
The RNAdvance Blood isolation kit purifies intact RNA suitable for downstream PCR-based applications.

Flexible and Scalable
Combining RNAdvance Blood chemistry with our Biomek Workstation offers a high throughput and minimal hand-on solution.

Product Specifications

Application Uses RNA Isolation, RNA Extraction, Nucleic acid sample prep, RNA Sequencing
Format Liquid
Starting Sample Material Blood
Automated Available Yes
Item Specifications Referenced A35604

Citations

Beineke, P., Fitch, K., Tao, H., Elashoff, M. R., Rosenberg, S., Kraus, W. E., . . . PREDICT Investigators. (2012, December 3). A whole blood gene expression-based signature for smoking status. BMC Medical Genomics, 5(58). doi:10.1186/1755-8794-5-58

  • Used to extract RNA from PAXgene-collected blood samples

Allaire, N. E., Bushnell, S. E., Bienkowska, J., Brock, G., & Carulli, J. (2013, January 5). Optimization of a high-throughput whole blood expression profiling methodology and its application to assess the pharmacodynamics of interferon (IFN) beta-1a or polyethylene glycol-conjugated IFN beta-1a in healthy clinical trial subjects. BMC Research Notes, 6(8). doi:10.1186/1756-0500-6-8

  • Used to extract RNA from PAXgene-collected blood samples

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Documentation and Related Materials

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Documentos técnicos