Application Notes

The real-world benefits provided by our technologies are realized only through their application.

Our growing repository of application notes runs the gamut of topics—from cutting-edge research on genomic and biopharmaceutical therapies to total organic carbon analysis for modern water systems.

Please choose your topic of interest:

Title	Description
CytoFLEX {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Evaluation of an Automated Instrument for Viability and Concentration Measurements of Cryopreserved Hematopoietic Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Two important parameters for determination of deleterious effects of cellular processing on hematopoietic progenitor cells are cell viability and concentration. The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins Hospital evaluated the Beckman Coulter Vi-Cell automated instrument for the measurement of these two parameters.
Automated TruSeq RNA Sample Preparation from FFPE tissue specimens utilizing the Biomek FXp Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes automated RNA library construction using the Beckman Coulter Biomek FXP Liquid Handler from archival FFPE tissue specimens.
Analyzing the Effect of Pipette Tip Geometries on Fluid Velocity and Shear Strain Rate: Biomek Wide Bore vs. Standard Pipette Tips {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Robert D Lund, Beckman Coulter Life Sciences
Automated Sample Preparation for Cell-Based Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The automation of sample preparation and analysis workflows for a variety of common cellular assays.
Automated Size Selection using SPRIselect beads and the Biomek FX Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes the automation of the Size Selection using SPRIselect beads on the Biomek FX Liquid Handler.
Automation of NGS Sample Size Selection using SPRIselect {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	To streamline the NGS sample preparation process, Beckman Coulter has recently launched the SPRIselect Reagent Kit to allow NGS sample size selection to be automated on Biomek liquid handling platforms for high throughput and high efficiency sample preparations.
Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes results obtained using a liquid handling-based automation system for the sample preparation of compounds to be analyzed by LC-MS/ MS and applied to the monitoring of multiple classes of pharmaceutical and illicit drugs.
Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Three-dimensional (3D) cell cultures offer greater physiological relevance than monolayer cultures for cellular interaction studies and compound screens.
Automated Sample Preparation for Analysis of 25-OH Vitamin D3 and 25-OH-Vitamin D2 by LC/MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this work, we demonstrate the feasibility of automating the sample preparation for liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis of 25-OH-Vitamin D2 and 25-OH-Vitamin D3.
Automated XTT Assay for Cell Viability Analysis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cellular viability assays are valuable for numerous workflows—from establishing proliferation rates to drug toxicity screens.
Cell Counting Performance of Vi-CELL BLU Cell Viability Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note examines how the new Vi-CELL BLU performs within specifications so that any instrument variability can be eliminated from the sample measurement.
Considerations of Cell Counting Analysis when using Different Types of Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note reviews several different cell types and cell preparation approaches using standard cells.
Switching from Oil Testing to Water and Back using the HIAC 8011+ and PODS+ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note details the processes required when switching from water based sampling to oil based sampling and/or from oil based sampling to water based sampling in the HIAC 8011+ and HIAC PODS+
Matching Cell Counts between Vi-CELL XR and Vi-CELL BLU {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note explains the flexibility to adjust the cell type settings to match results obtained from an equivalent sample when run on the Vi-CELL XR on the Vi-CELL BLU.
Red Blood Cell Microparticle Analysis by Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Discover how to stain microparticles for flow cytometric analysis. The assay describes sample preparation, staining, and gating steps to distinguish red blood cell-derived microparticles from microparticles from other tissue origins. Representative data from several replicates are provided in order to report out precision obtainable from this approach.
Single Cell Analysis of Autophagy Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Provides a detailed procedure for using the Cyto-ID Autophagy kit in a flow cytometry assay. Contains background information on the physical and chemical hallmarks of autophagy and the benefits of a single cell assay versus bulk analysis of this biological process.
Competitive Mouse Bone Marrow Transplantation Reconstitution Assay Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Using primary-conjugated antibodies against CD45.1 and CD45.2, we measure competitive bone marrow transplantation success by quantifying the proportion of donor cells (marked by CD45.1/Ly5.1 expression) and recipient cells (marked by CD45.2/Ly5.2 expression) in the peripheral blood of irradiated recipient mice post-transplantation.
Calcium Flux Assay by Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The unique peristaltic sample delivery system of the CytoFLEX analyzer enables optimized measurements of transient changes in intracellular calcium in cells following agonist activation.
Flow Cytometric Analysis of Auto-fluorescent Cells in Marine Demosponge Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Natural autofluorescence of marine organisms can be detected in flow cytometers. Utilizing this, an assay to assess aggregation and reorganization of the sponge is described.
Using Flow Cytometry to Assess Protein Aggregation in Bacterial Overexpression Systems Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes the use of Proteostat dye in a flow cytometric assay for detection of aggregated protein in bacteria inclusion bodies. This assay can be used to assess bacterial culture conditions that favor production of soluble protein. This method reduces the amount of sample preparation required compared to gel electrophoresis-based methods.
Measuring Proliferation in Leukemia Cell Lines Using CFSE Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye which is cell permeable. It binds covalently to intracellular proteins. Fluorescence decreases over time as the dye is divided between daughter cells at each division. In this application note a detailed protocol for using this assay to assess cytotoxic effects of a pharmacologic agent is described.
21 CFR Part 11 Data Integrity for On-line WFI Instruments {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This whitepaper takes a look at how quality-critical on-line Total Organic Carbon (TOC) and conductivity instrumentation for WFI (water for injection) quality control can be configured to help companies comply with the FDA’s expectations on data integrity, including the use of Microsoft Active Directory and PDF data export.
Illumina TruSeq Exome Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq Exome Library Prep kit is a workflow to generate indexed paired-end libraries and is specially designed to work with Formalin Fixed Paraffin Embedded (FFPE) samples
A Fully-Automated, Image-Based Screen for miRNAs that Regulate Cancer Cell Viability and Chemosensitivity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist, \| Beckman Coulter Life Sciences, Indianapolis, IN \| Tammy Chan, Field Application Scientist, \| Molecular Devices, Sunnyvale, CA \| Kristin Prasauckas, Manager, Field Applications Scientist, \| Molecular Devices, Sunnyvale, CA \|Tara Jones-Roe, Marketing Manager, \| Beckman Coulter Life Sciences, Indianapolis, IN
FormaPure DNA FFPE Extraction Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Increasingly, Formalin-Fixed, Paraffin-Embedded (FFPE) samples are an essential part of many next-generation sequencing (NGS) sample prep workflows, specifically in the field of cancer research
LS13320XR Laser Diffraction Particle Size Distribution Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sample Preparation – How to measure success!
Agencourt RNAdvance Blood Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Supplemental protocol for micro RNA and total RNA isolation from PAXgene preserved blood.
CD6: New Low/Negative Surface Marker for Human FOXP3+ Naturally-occurring Regulatory T-cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Carlos A. Garcia Santana M.D., Ph.D. \| James W. Tung Ph.D. \| Sergei Gulnik Ph.D.
Evaluation of Instrument to Instrument Performance of the Vi-CELL BLU Cell Viability Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note examines the comparability of results from multiple Vi-CELL BLU cell viability analyzers.
Automated FFPE DNA Extraction using the FormaPure DNA Extraction Kit using the Biomek Series of Automated Liquid Handlers from Beckman Coulter, Inc. {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. \| Beckman Coulter, Inc. \| Published 2016
Secondary Screening Solutions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	For drug discovery and biomarker research, our series of Biomek automated workstations provide comprehensive tailored solutions for your high throughput screening workflows
Automation of RNA Isolation from Blood Samples stabilized in RNAgard® Blood Tubes, using the Magnetic Bead-Based Agencourt RNAdvance Blood System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Clinical Research studies often require blood sample collection at multiple geographic sites under a wide range of conditions
Advancing Science is in our DNA {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	From the Human Genome Project to today’s quest for precision medicine, we’ve long invested in providing genomic products that reduce complexity and improve productivity
Automated DNA Library Construction using the NEBNext® Ultra II DNA Library Prep Kit for Illumina® on the Beckman Coulter Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith, M.S. \| Beckman Coulter, Inc. Daniela Munafo, Ph.D. \| New England Biolabs, Inc. Brad Langhorst, Ph.D. \| New England Biolabs, Inc. Laurie Mazzola \| New England Biolabs, Inc. Joanna Bybee \| New England Biolabs, Inc. Danielle Rivizzigno \| New England Biolabs, Inc. \| Published 2016
Automation of the Illumina® TruSeq® DNA PCR-Free Sample Preparation Kit on the Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. Beckman Coulter Life Sciences, Indianapolis, IN USA \| Published 2014
Agencourt DNAdvance System Nucleic Acid Extraction from Mammalian Tissue Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Built on SPRIB paramagnetic bead-based technology, the Agencourt DNAdvance kit is a high throughput genomic DNA (gDNA) isolation reagent kit enabling the purification of high quality DNA from mammalian tissue samples
Vi-CELL BLU Regulatory Compliance - 21 CFR Part 11 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The following document describes the relevant portions of the 21 CFR Part 11 regulations and their implementation using the Vi-CELL BLU control software.
Root cause investigation for pharmaceutical water systems	In this whitepaper we discuss some typical sources of contamination in water systems and how root-cause investigations can be supported using on-line Total Organic Carbon (TOC) analysers.
Cell Culture Monitoring with the Vi-CELL MetaFLEX Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note that details how the Vi-CELL MetaFLEX is an effective tool for analyzing mammalian and insect cell culture media.
Internal results based on Normal whole blood {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Internal results based on Normal whole blood
Simple, Yet Effective – Automating Bradford Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Excellent consistency of automated standard dilution and Bradford assay preparation
Analyzing Mussel/Mollusk Propagation Using the Coulter Analyzer Multisizer 4e {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note details how the use of a Coulter Principle based instrument provides a major step forward in accurately verifying both the size of the feed and concentration for a fish hatchery.
Automated Illumina TruSeq Custom Amplicon Low Input Library Preparation Kit on the Beckman Coulter Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. \| Beckman Coulter, Inc. \| Published 2016
Workflow protocols {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Workflow protocols
ZAP-70 assessment by flow Cytometry: improving assay sensitivity, robustness and workflow using an isoclonic control and PerFix-nc. {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	ZAP-70 assessment by flow Cytometry: improving assay sensitivity, robustness and workflow using an isoclonic control and PerFix-nc.
Quantification of Cellular Toxicity with Particle Characterization and Centrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Beckman Coulter tools enable quantification of cellular toxicity caused by nanoparticles, reducing time to remove aggregated particles and quantifying cell toxicity in the presence of single-walled carbon nanotubes.
Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube based on DURAClone dry reagent technology {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube based on DURAClone dry reagent technology
Application note for advanced analysis of data obtained with DURAClone RE CLB {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note for advanced analysis of data obtained with DURAClone RE CLB
Application note for advanced analysis of data obtained with DURAClone RE PC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note for advanced analysis of data obtained with DURAClone RE PC
Custom Design Service Reagents: Recommendations on Lysis buffers and Protocols {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Custom Design Service Reagents: Recommendations on Lysis buffers and Protocols
FoxP3 staining in human whole blood using PerFix-nc {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	FoxP3 staining in human whole blood using PerFix-nc
Rapid and easy intracellular + surface staining with PerFix-nc_MouseMarkers {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Rapid and easy intracellular + surface staining with PerFix-nc_MouseMarkers
PerFix-nc protocol for PBMC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	PerFix-nc protocol for PBMC
Automated ELISAs—Save Time and Increase Throughput {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We demonstrate how the integration of the MultiWash™+ Microplate Washer and EMax® Plus Microplate Reader to a Biomek NXP Workstation facilitates the automated processing and analysis of ELISAs.
Automating Bradford Assays—Reliable Results with Less Effort {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this work we demonstrate the automated preparation and analysis of Bradford protein assay samples using a Biomek NXP Workstation with an integrated EMax® Plus Microplate Reader.
Automated Optimization of Cell Transfection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we demonstrate how the Biomek FXP Workstation can be used to identify the optimal conditions for siRNA transfection in a single experiment.
Automation of Multi-Day Cellular Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we demonstrate the use of SAMI Process Management software to schedule and execute the automated liquid handling steps on a Biomek SAMI EX Workstation, for overlapping 8-day embryonic stem cell differentiation experiments.
Automated Liquid Handling for Cell Staining and Imaging {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we describe a variety of automated solutions to reduce the time, effort, and complexity associated with sample preparation workflows for cell imaging.
Automation of the Illumina® TruSeq® DNA PCR-Free Sample Preparation Kit on the Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S.
Automated Illumina TruSeq Nano DNA Sample Preparation on the Biomek 4000 Laboratory Automated Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: David Horvath, M.S. - Beckman Coulter, Inc. \| Published 2016
Thirteen Color Immunophenotyping T Cell Subsets in Whole Blood Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Contains a comprehensive overview of T cell biology and the implications for multicolor immunophenotyping panel design. Includes a detailed gating strategy for deep immunophenotyping of T cell populations including rare cell types. Also includes step-by-step instructions for setting up the CytoFLEX for multicolor immunophenotyping including use of the Compensation Library.
Reproducible and Efficient Automation of DTR Purification Using the Biomek 4000 Workstation and CleanSEQ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt CleanSEQ* application for the Biomek 4000 Laboratory Automation Workstation is a rapid, high performance dye-terminator removal method utilizing Solid Phase Reversible Immobilization (SPRI) paramagnetic beadbased technology.
Biomek Automated 384-well qPCR Setup using KAPA Biosystems Library Quantification Kit for Illumina Sequencing Platforms {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Library quantification is an essential step in the illumina next-generation sequencing(NGS) workflow
Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Michael Kowalski, Staff Applications Scientist \| Beckman Coulter Life Sciences, Indianapolis, IN \| Published 2016
High-Throughput Illumina® TruSeq® Nano DNA Library Construction on the Biomek FXP Dual-Arm Multi-96 and Span-8 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The manual preparation of large numbers of sequencing libraries, which can be labor-intensive and time-consuming, is a bottleneck for many laboratories
Automation-Biomek-i7-application-note-cell-line-development-selection-enrichment {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Cell Line Development – Hit Picking {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note we discuss how hit picking was automated as part of a cell line development workflow
Application Note: Advanced analysis of data obtained with DuraClone RE PC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note for advanced analysis of data obtained with DuraClone RE PC
High Recovery of Nucleic Acids withSuperior Yield and Purity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt DNAdvance system, nucleic acid extraction from mammalian tissue samples.
Analyzing the Effect of Pipette Tip Geometries on Fluid Velocity and Shear Strain Rate: Biomek Wide Bore vs. Standard Pipette Tips {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Robert D. Lund, Beckman Coulter Life Sciences, Indianapolis, IN \| Published: 2016
Illumina TruSeq Stranded mRNA Sample Preparation Kit Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Illumina TruSeq® Stranded mRNA Sample Preparation Kit protocol converts mRNA from total RNA into a template sample of known origin
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAdvance Tissue Kit uses Beckman Coulter’s patented Agencourt SPRI paramagnetic bead-based technology to isolate total RNA (microRNA and mRNA).
Particle Characterization - Nanotube Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Carbon nanotubes are an emerging material that has had increasing industrial application in material reinforcement and great future potential in electronics, energy storage and conversion, and medicine
MoFlo™ Bacterial Proliferation and PHB Production {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Population dynamics were follow ed during growth on phenol in response to adaptation processes of Cupriavidus necato, bacterial individuals morphologically nearly uniform, yet structurally and functionally diverse
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek NXP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Amy Yoder, Michael Kowalski Ph.D., Li Liu Ph.D., Laura Pajak Ph.D. \| Life Science Automation, Beckman Coulter Inc., Indianapolis, IN \| Published 2011
MoFlo™ Fluorescence Resonance Energy Transfer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Fluorescent proteins have become a mainstay in the study of protein localization, protein-protein interaction and gene expression
MoFlo™ Chromosome Sorting {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Flow cytometric chromosome classification (flow karyotyping) has been routinely used in the analysis of mammalian karyotypes as well as to assess chromosomal abnormalities
MoFlo Astrios EQ Enhanced Forward Scatter: Simultaneous Sorting of Micro- and Macro- Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The MoFlo Astrios EQ series provides solutions to investigate biological materials between 200 nm and 30 μm
High Purity Simultaneous Cell Sorting of Nano and Large Phytoplankton Using the MoFlo Astrios EQ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Phytoplankton conversion of light on the upper limits of the ocean consists of half of the photosynthesis on the Earth
Background Subtraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	When measuring a water sample to quantify TOC (Total Organic Carbon), most TOC analyzers add some combination of reagents and dilution water to the initial sample in order to complete the measurement
Issues with Testing Jet Fuels for Contamination! {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Joe Dabbs \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
The Coulter Principle for Outlier Detection in Highly Concentrated Solutions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Particle characterization techniques generally fall into one of two categories: “ensemble” techniques (multiple par ticles are analyzed simultaneously), or “discrete” techniques (individual particles are measured one at a time)
Inorganic Carbon Removal {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	A water sample initially contains two types of carbon: Total Inorganic Carbon (TIC) (from CO2 gas dissolved in H2O and dissolved carbonates in the water) and Total Organic Carbon (TOC) (from organic species)
8011+ Web Server Application Setup Procedure {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Application Scientist for Beckman Coulter Life Sciences Company in Grants Pass, Oregon, USA.
Techniques of the Coulter Principle and Dynamic Light Scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Liposomes are special lipid vesicles ranging from 50 nm to 5 μm in diameter
Using Laser Diffraction Analysis in Pigment Sizing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Pigments and paints are an important class of industrial materials
Calibration {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The calibration of a Total Organic Carbon (TOC) Analyzer is a crucial part of ensuring a quality measurement
Contamination Monitoring in the Hydraulics Industry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Dave Dunham \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
Sieves to Laser Diffraction...Hours to Seconds. See What You've Been Missing! {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Published 2013
Cell Separation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	During various stages of the cell culturing process, centrifugation is frequently used to isolate extracellular products or to separate cells from their aqueous environment
Optimizing Cell Separation with Beckman Coulter's Centrifugal Elutriation System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In many applications of cell biology, separation of cells from a heterogenous population to an enriched population of specific target cells is necessary to answer relevant scientific questions.
Density Gradient Separation of Gold Nanorods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Gold Nanorods (AuNR) hold great promise for biomedical imaging
Analysis of Membrane Protein Dimerization by Sedimentation Equilibrium {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Karen G. Fleming - Department of Molecular Biophysics and Biochemistry, New Haven, CT
Chymotrypsin: Characterization of a Self-Associating System in the Analytical Ultracentrifuge {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Paul Voelker and Don McRorie \| Beckman Coulter
Exosome-Depleted FBS Using Beckman Coulter Centrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph. D. \| Beckman Coulter Life Sciences, Indianapolis, IN
High-Performance Exosome Purification and Characterization via Density Gradient Ultracentrifugation and Dynamic Light Scattering {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Exosomes purification and analyses comprise a fast evolving research area; more than 70% of published research on exosomes has been done within the last six years
Efficient Kit-Free Nucleic Acid Isolation uses a Combination of Precipitation and Centrifugation Separation Methods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Julia P Luciano-Chadee, Beckman Coulter, Inc., Indianapolis, IN 46268 \| Published 2017
Get Control in GMP Environments {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Floor-standing ultracentrifuges and high-performance centrifuges are vital to many bioproduction processes
A Standardized, Automated Approach for Exosome Isolation and Characterization using Beckman Coulter Instrumentation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph. D., Zach Smith, M.S. Beckman Coulter Life Sciences, Indianapolis, IN 46268
Rapid Determination of Spectrophotometric Absorptivity by Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: R. A. Gray, A. Stern, T. Bewley, and S. J. Shire Genentech, Inc. \| South San Francisco, CA
Lentiviral Vector Preparation Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Viral vectors are one of the most commonly used tools for genetic manipulation in mammalian cells
Enhancing Vaccine Development and Production {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, PhD, Senior Application Scientist \| Beckman Coulter, Inc., Life Sciences, Indianapolis, IN USA \| Published 2015
Short Column Sedimentation Equilibrium Analysis for Rapid Characterization of Macromolecules in Solution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Thomas M. Laue University of New Hampshire Department of Biochemistry Durham, NH 03824
Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, PhD, Application Scientist \| Beckman Coulter, Inc., Indianapolis, IN USA \| Published 2014
Avanti JXN Series - Complete Solution for Protein Purification Workflow {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Purified proteins are required for many proteomics applications such as X-ray crystallography, NMR, mass spec, and in vitro biochemical assays
Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Chad Schwartz, Ph.D., Dean Clodfelter, M.S. \| Beckman Coulter, Inc., Indianapolis, IN 46268
High-Performance Automation and Centrifugation Preparation of Carbon Nanotubes for Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Single-Walled Carbon Nanotubes (SWCNT) experienced increasing interest in the past 15 years for semiconductors, fuel cells, and biomedical applications
Beckman Coulter’s New Line of Centrifuges Provides Support for 21 CFR Part 11 Compliant Laboratories {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As technology and software advance, security and protection of data is becoming increasingly important to ensure a properly functioning lab environment
Linearity of BSA Using Absorbance & Interference Optics {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dean Clodfelter, M.S., \|Chad Schwartz, Ph.D., Beckman Coulter Inc., 5350 Lakeview Parkway Dr., Indianapolis, IN 46268
The Lipemic Serum Clarification System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Lipemic Serum Clarifi cation System clears blood samples of lipemia caused by chylomicrons—fat particles 80–500 nm in diameter that impair accuracy of spectrophotometric analyses
Particle Characterization & Centrifugation - Nanotoxicity Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nanotechnology biomedicine is an emerging field still in its nascent phase
The Solution Conformation of Novel Antibody Fragments Studied Using the Proteomelab XL –A Analytical Ultracentrifuge {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Peter J. Morgan, Olwyn D. Byron, Stephen E. Harding \| Department of Applied Biochemistry and Food Science \| University of Nottingham, Sutton Bonington, U. K.
Stability of the Human Immunodeficiency Virus-1 Reverse Transcriptase Heterodimer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Jacob Lebowitz - University of Alabama, Birmingham, AL
The Sizing of Non-Spherical, Sub-Micron Particles using Polarization Intensity Differential Scattering (PIDS™) {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nearly all laser-based particle-sizing devices make no allowance for the shape of the materials under test, regardless of the size of the particles
Particle Characterization & Centrifugation - Nanotoxicity Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Nanotechnology biomedicine is an emerging field still in its nascent phase.
How to Characterize Multimodal Samples using Particle Characterization Equipment {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note will discuss the topic of size resolution in particle characterization and advantages and disadvantages of various techniques
Particle Characterization - Flow Pressurization Eliminates Bubbles for More Accuracy {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Bubbles create major issues when characterizing nanoparticles in solution because the characterization techniques typically utilize light scattering specifically when determining size distribution and stability
Method Overview {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	A water sample initially contains two types of carbon: Total Inorganic Carbon (TIC) (from CO2 gas dissolved in H2O and dissolved carbonates in the water) and Total Organic Carbon (TOC) (from organic species)
Achieving Compliant Batch Release - Sterile Parenteral Quality Control {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Tony Harrison, Compliance and Applications Specialist \| Beckman Coulter, Inc., United Kingdom \| Published 2016
Using The Agencourt ® AMPure® And Agencourt CleanSEQ® Methods To Manage A Variety Of Input Sample Quality {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we present sample purification methods that assist in addressing the varying input sample quality encountered by facilities performing PCR amplification and sequencing for SNP detection.
Minimizing process variability in the manufacturing of Bottled Drinking Water {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note by Bill Bars detailing the use of the HIAC 8011+ for variability testing of bottled drinking water.
Eight Color Immunophenotyping Unlysed Whole Blood Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This whole blood assay does not involve harsh lysis or centrifugation steps, minimizing any cell perturbations from sample handling. This 8-color panel provides a broad survey of peripheral blood leukocytes.
CytoFLEX Application Note18 ParameterFinal0319 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
The MD Anderson SPRI8 Experience: Using Agencourt8 CleanSEQ8 and Agencourt AMPure8 in HLA Sequencing-Based Typing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Cano, Pedro \| Fernandez-Vina, Marcelo \| Guerrero, Edward \| Barnes, Titus \| Partlow, David \| Willis, Dana \| Zhao, Weicheng \| Patel, Hemantkumar \| Heins-Israel, Karen \| EL-Fahmawi, Bassam
Mo Bio PowerMag Soil DNA Isolation Kit on Biomek 4000 Liquid Handling Automation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Shilpa Parakh, Beckman Coulter, Inc \| Published 2016
Agencourt® FormaPure® for miRNA and Total RNA {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This protocol describes the procedure for purifying miRNA, including total RNA, from formalin-fixed, paraffin embedded (FFPE) tissue sections
Continuous Cell Culture {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Continuous cell culture provides scientists the ability to readily have assay-ready plates available to test potential agents or to have large quantities of multiple cell lines available for therapies research.
FormaPure DNA Extraction kit for FFPE Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The FormaPure DNA extraction kit provides a convenient extraction process utilizing Beckman Coulter’s proprietary solid phase reversible immobilization (SPRI) chemistry to extract DNA from FFPE curls.
Application note: Advanced analysis of data obtained with DuraClone RE CLB {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note for advanced analysis of data obtained with DuraClone RE CLB
Leveraging the Vi-CELL MetaFLEX for Monitoring Cell Metabolic Activity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note about the use of the Vi-CELL MetaFLEX for detecting small changes in metabolic activity in different cell types.
Vaporized Hydrogen Peroxide Decontamination of Vi-CELL BLU Instrument {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the method and results for testing the Vi-CELL BLU for VHP resistance.
Automating Cell Line Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features automating cell line development with a Biomek i7 liquid handling workstation and integrated devices in order to overcome challenges for this workflow
Application Note: Compensation setup for high-content DURAClone reagents {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Compensation setup for high-content DURAClone reagents
Multi-Wavelength Analytical Ultracentrifugation of Human Serum Albumin complexed with Porphyrin {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Cell Line Development – Limiting Dilution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note we describe the automation of limiting dilution on a Biomek i7 liquid handling workstation that overcomes a number of challenges associated with this workflow
A workflow for medium-throughput isolation of cfDNA from plasma samples using Apostle MiniMax™ on the KingFisher™ Technology {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the use of Apostle MiniMax™ High Efficiency cfDNA Isolation Kit, in conjunction with the KingFisher Duo Prime automated protein purification system.
Application Note: Advanced analysis of human T cell subsets {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13-color tube based on DURAClone dry reagent technology
Reproducible and Efficient Automation of PCR Reaction Setup and Purification Using the Biomek 4000 Workstation and AMPure XP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Ruth Zhang, Li Liu, Amy Yoder, John Palys, Bryan Daniels, Isabel Gautreau and William Godfrey Beckman Coulter Life Sciences, Indianapolis, IN \| Published 2013
High Recovery of DNA for Downstream Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt Genfind v2 DNA purification system isolates and purifies genomic DNA (gDNA) from whole blood and serum
Automated Solutions for Cellular Analysis Through Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski - Staff Applications Scientist, Li Liu - Senior Development Scientist, Amy Yoder - Staff Development Scientist, Beckman Coulter Life Sciences, Indianapolis, IN \| Published 2014
Automation of Cell Staining Using the Biomek 4000 Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Manual cell staining of cardiomyocytes, either to characterize antigen expression or to assess cell health, is labor-intensive and time-consuming.
Automated Data Management for Reliable Cell-Based Screening {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist \| Beckman Coulter Life Sciences, Indianapolis, IN Timothy Sherrill, Staff Systems Engineer \| Beckman Coulter Life Sciences, Indianapolis, IN Tara Jones-Roe, Marketing Manager \| Beckman Coulter Life Sciences, Indianapolis, IN
High Quality RNA from Preserved Blood {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt RNAdvance Blood System, RNA extraction from RNA-stabilizing blood collection tubes.
LS 13 320 XR Vs. Malvern Mastersizer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Industrial Application side by side comparison running a High Purity Carbon
Automated Optimization of Cell Transfection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Demonstrated application on how the Biomek FXP Workstation can be used to identify the optimal conditions for siRNA transfection in a single experiment.
Flexible ELISA Automation with the Biomek i5 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Automated ELISA assay workflow \| Optimized liquid transfers eliminate the need for a plate washer \| High-capacity deck accommodates fresh tip usage
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
The new LS 13 320 XR Extended Range 10 nm - 3500 μm {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Application note exhibiting the extended range of the LS 13 320 XR
AGENCOURT® RNAdvance® CELL v2 Supplemental Protocol {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	For micro RNA and total RNA isolation from cultured cells.
Identification of Natural Killer Cells in Rat Placenta Model of Preeclampsia Using Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	A special population of natural killer cells (uNK) has been implicated in preeclampsia. uNK cells have contact with the trophoblast, modify uterine arteries to promote blood flow, and modulate trophoblast invasion in a dose-dependent manner. In this application note a detailed method for preparing resident NK cells from tissue and an assay to access NK cell activation is provided.
Phagocytosis of Bacteria in a Whole Blood Assay Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes an opsonization assay which can be used to assess the phagocytic capabilities of immune cell in whole blood. The benefits of using multicolor flow cytometry for the assay is that different phagocytic potential across various immune cell types can be determined in the same sample.
Using Cell Impermeant DNA Binding Dyes for Viability Assessment in Multicolor Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Compares viability staining using DRAQ7, DAPI, and 7-AAD in a multicolor immunophenotyping panel. Attributes of each dye are evaluated to aid in selection during panel design.
CytoFLEX Instrument Set-up for Measuring Extracellular Vesicles Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes the instrument set up and verification steps required for measuring extracellular vesicles by flow cytometry. Instructions for reducing background particles found in buffers and reagents to enhance small particle detection.
Illumina TruSight® HLA Sequencing Panel Automated on the Biomek FXP HLA SP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith, MS, Senior Applications Scientist - Beckman Coulter, Inc. \| Nate Baird, PhD, Scientist, Illumina \| Brad Baas, PhD, Senior Scientist, Illumina \| Published 2015
Automated Salt-Assisted Liquid-Liquid Extraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski and Ruth Zhang \| Beckman Coulter Life Sciences, Indianapolis, IN, USA, Yun Yun Zou and Michael Jarvis \| SCIEX, Concord, ON, Canada
Agencourt RNAClean XP System - Enzymatic Purification System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAClean XP kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic reactions such as cDNA synthesis and in vitro transcription (IVT) reactions
Automated Optimization of IgG Production in CHO Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist - Beckman Coulter Life Sciences, Indianapolis, IN \| David Apiyo, Marketing Applications Manager - Pall ForteBio, Fremont, CA \| Rashi Takkar, Product Manager - Pall ForteBio, Fremont, CA \| Tara Jones-Roe, Marketing Manager -Beckman Coulter Life Sciences, Indianapolis, IN
Stem Cell Analyses Using The Vi-CELL™ XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Stephen E. Szabo, Ph.D. \| Sarah Monroe, B.S.
Automated DNA Library Construction Using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® on the Beckman Coulter Biomek FXP Automated Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We describe the automation of the New England Biolabs NEBNext Ultra DNA Library Preparation Kit for Illumina on the Beckman Coulter Biomek FXP Dual-Arm Multichannel 96 and Span-8 automated liquid handler (Biomek FXP ).
Scalable, Automation-friendly and High Throughput PCR Purification {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt CleanSEQ system, dye terminator removal.
High-Capacity Next Generation Sequencing Platforms {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Meet the Demand of Illumina’s High-Capacity NGS Platforms with Biomek Automated Sample Preparation
The New Avanti J-15 Centrifuge Improves Sample Protection and Maximizes Sample Recovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	By focusing on sample protection, the Avanti J-15 series has reduced sample disturbance during acceleration and deceleration, increasing sample yield, decreasing contamination and increasing centrifugation efficiency.
The New Avanti J-15 Centrifuge Time Saving Deceleration Profile Improves Workflow Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The implementation of Ultra Harmonic Technology into the Avanti J-15 series renders our centrifuge ideal for applications that require multiple runs. It saves up to 10% of instrument usage time for a single application and the time savings maximizes overall laboratory assent management.
Enumeration and Size Distribution of Yeast Cells in the Brewing Industry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Steve Szabo, Ken Lindo, Brendan Yee \| Cellular Analysis Business Center, Beckman Coulter Inc, Miami, FL
Determination of Size and Concentration of Particles in Oils {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The concentration and size distribution of particles in an oil sample may be measured using the Coulter Principle also known as the Electrical Sensing Zone (ESZ) method
Evaluating Contact Lens Disinfection Efficacy Using the Beckman Coulter™ Multisizer™ 3 COULTER COUNTER® {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The percentage of aggregates in bacteria cultures may be determined by employing the Coulter Principle, also known as Electrical Sensing Zone (ESZ) technique
Beer, Evaluation of Final Product and Filtration Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The concentration and size distribution of particles in beer may be measured using the Coulter Principle also known as the Electrical Sensing Zone (ESZ) method
Using Pressure to Reduce Bubble Contamination from Particle Count Results {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Dave Dunham \| Beckman Coulter, Inc, Grants Pass, OR \| Published 2016
Using Laser Diffraction in Folk and Ward Phi Notation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Soil studies span a range of applications including agriculture, architectural planning, and historical environmental studies
Using the DelsaMax CORE for Molecular Weight Analysis - Protein Sizing Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Accurate determination of protein and antibody molecular weight has great impor tance in the field of molecular biology
USP System Suitability {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The US Pharmacopeia (USP 36-NF 31, section <643>) provides specific guidance on how to qualify TOC instrumentation for use
Coulter Principle Short Course {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	What is a particle? According to Webster’s Dictionary a particle is “a minute quantity or fragment” or “a relatively small or the smallest discrete portion or amount of something
HIAC 8011+ Using Pressure To Reduce Entrained Air from Fluid Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Dave Dunham \| Global Marketing Manager for Beckman Coulter Life Sciences \| Published 2013
Industrial Application Note 8011+ Reporting Standards Feature and Synopsis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Application Scientist for Beckman Coulter Life Sciences Company in Grants Pass, Oregon, USA.
Determine Hydrodynamic Size of Surfactant Micelles with DelsaMax CORE {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sodium Dodecyl Sulfate (SDS) is a commonly used amphiphilic surfactant with wide-ranging applications, including chemical disinfection to protein denaturation in SDS-PAGE gels, cell lysing for DNA extraction, and nanoparticle solubilization
Biological Applications for Multisizer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Aspirin Analogues and Flow of Erythrocytes through Narrow Capillaries
Optimizing the HIAC 8011+ Particle Counter for Analyzing Viscous Fluids {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Sr. Application Scientist for Beckman Coulter Life Sciences in Grants Pass, Oregon, USA \| Published 2013
The Particle Analyzers Advancing Life Science Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Scientists share how automated cell counting is revolutionizing research
21CFR part 11 Data Integrity for On-line WFI Instruments	Whitepaper by author Tony Harrison, a Compliance and Applications Specialist for Beckman Coulter Life Sciences \| Published 2017
Quality Control Electronic Records for 21 CFR part 11 Compliance	This paper describes how Quality Control instruments can be optimised for pharmaceutical use, helping to improve the integrity of the data in the final electronic record.
Specifying Non-Viable Particle Monitoring for Aseptic Processing	This paper details key considerations when implementing a continuous non-viable particle monitoring (NVP) system.
Automating Biopharma Quality Control to Reduce Costs and Improve Data Integrity	This whitepaper takes a look at four common quality control procedures and how automation can help improve data integrity by reducing opportunities for human error, whilst also saving time and reducing operating costs. The four quality control procedures discussed in this paper form a common thread in the biopharmaceutical manufacturing industry and are water quality testing, cleanroom routine environmental monitoring, biological cell counting and viability testing and final injectable drug particulate testing.
Avoid the Pitfalls when Automating Cell Viability Counting for Biopharmaceutical Quality Control	In this paper we show one example of how identifying viable cells by minimum diameter alone can introduce variability of circa 33% in the reported cell concentration. This paper describes how to avoid these common pitfalls when user is adopting a new automated method of cell counting.
Optimizing Workflow Efficiency of Cleanroom Routine Environmental Monitoring {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper examines a thoughtfully designed and well-executed environmental monitoring program that tests the controls put in place to maintain the cleanroom to the required Good Manufacturing Practices standards (e.g., CGMP, EU-GMP, and PIC/S).
Assessing T Cell Subpopulations by TCR Expression in PBMCs Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We outline a protocol using DURAClone™ IM panels, which are unitized, dry format, room temperature stable reagent cocktails, to delineate and quantify T cell subpopulations from a heterogeneous sample of isolated normal PBMCs based on TCR composition.
Vi-CELL BLU FAST Mode Option {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note explains the optional FAST Mode of operation in the new Vi-CELL BLU which can be used in either plate or carousel configuration to significantly reduce sample to sample operation times.
Compensation setup for high content DURAClone reagents {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Compensation setup for high content DURAClone reagents
Efficient Factorial Optimization of Transfection Conditions {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Design of Experiment (DOE) approach can be quickly automated on the Biomek i7 Automated Workstation to identify optimal transfection conditions for a variety
Automating Biologics Signature Peptide Quantitation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Ian Moore, Technical Marketing Manager \| SCIEX, Concord, Canada, Michael Kowalski, Staff Applications Scientist \| Beckman Coulter Life Sciences, Indianapolis, IN
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Extraction of high quality DNA from formalin fixed paraffin embedded (FFPE) tissue is essential
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
Biomek FXP Laboratory Automation Workstation as a Tool for Discovering Targets & Compounds for Host-Directed Influenza Therapy {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Markus Lesch, Project Manager, Center for Systems Biomedicine, Steinbeis Innovation gGmbH. Falkensee, Germany, Alexander Karlas, Senior Scientist, Max Planck Institute for Infection Biology, Berlin, Germany. Published: 2015
HIAC Industrial - Our overview solution for fluid power testing for all applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note details measuring fluid contamination by cleanliness standards like ISO 4406, SAE AS4059, and NAS 1638 for oil and hydraulic fluid applications.
Ten Color Immunophenotyping of Mouse NK Cells Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Surface-marker expression is critical to the delineation of NK cell maturity, function, and localization. We present a protocol and gating strategy for the identification of splenic murine NK cell populations via the CytoFLEX flow cytometer using a 10-color screen.
Identification of Circulating Myeloid Cell Populations in NLRP3 Null Mice Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this case study, using the surface markers Ly6C (hematopoietic precursor cells, monocytes), Ly6G (granulocytes/neutrophils), and CD115 (macrophage colony-stimulating factor [M-CSF] receptor; macrophages), we examined and identified the differences in circulating cell populations in fresh blood samples.
Flow Cytometric Quantification to Assess Doral Endocytosis Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the use of flow cytometry to examine the phagocytosis of collagen type I-coated fluorescent microspheres by rat embryonic fibroblasts. Cellular uptake can be assessed quickly in a large number of cells using flow cytometry.
Scalable Plasmid Purification using CosMCPrep {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the flexibility of CosMCPrep, a scalable plasmid purification kit. CosMCPrep is shown here to work with volumes up to 5.5 mL purifying up to 300 µg of plasmid.
Characterizing T Helper Cells Populations by Intracellular Cytokine Expression Using Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	T helper cells are distinguished functionally by their cytokine profile, their major effector molecules. This application note provides a procedure for assessing the cytokine profile using flow cytometry and demonstrates T Helper cell subtypes Th1, Th2, Th17 and a non-classic Th1 type.
Phenotypic Characterization of Human Natural Killer Cell Populations in Peripheral Blood Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes a 10 color, 13 marker panel to investigate the NK cell population, both phenotypically and functionally in human PBMC samples. The panel includes the use of an exclusion markers on a dump channel used to gate out non-NK cells. Deep phenotyping markers for the NK cell compartment are included and a comprehensive gating strategy is defined.
Identifying Discrete Mouse Splenocyte and Peripheral Blood Cell Subpopulations Using Surface Markers Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Flow cytometry is extremely useful for detecting cell surface antigen expression, and thereby facilitates the characterization, identification, and isolation of discrete cell populations within heterogeneous samples. We describe a method to rapidly isolate and delineate CD3ε-positive T cell and CD19-positive B cell populations from murine spleen and peripheral blood samples.
Identifying Brain Tumor Initiating Cells Using Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Tumors represent a heterogenous population. Enumerating cells with stem cell like properties via co-expression of markers provides insight into the evolution of the tumor over time and throughout treatment. Detailed procedure for processing brain tissue for culture and flow cytometric analysis is provided.
Plant Genome Analysis by Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Provides a detailed protocol for preparing nuclei from plant tissues. DNA in the nuclei is then stained with propidium iodide and the flow cytometer is used to assess DNA content. Values obtained are then correlated with the Kew Database to assess ploidy. This assay can be used to investigate the role of endoreplication in plant development across species.
Flow Cytometric Approach to Probiotic Cell Counting and Analysis Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Describes a staining procedure and instrument set up for absolute counting of probiotic bacterial culture. A workflow comparison with the traditional plate-based assay as well as correlated cell counts are provided.
Centrifuge-application-note-intrinsically-disordered-protein-AUC {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Isolation of DNA from Buccal Swabs using DNAdvance Kit	This protocol increases the volume of lysis buffer so that these two swabs from a single individual can be run in a single well and have the swab heads covered by lysis buffer.
Multi-Wavelength Analytical Ultracentrifugation of Human Serum Albumin complexed with Porphyrin {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We show here how one can take advantage of the in Sorét band of porphyrins complexed with different cations to spectrally deconvolute dissimilar optical properties from different molecules.
Q&A on Detecting Moisture in Hydraulic Fluid, Oil and Fuels {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Monitoring the amount of moisture in petroleum based fluids before 100% saturation occurs will reduce degradation and will increase the life and performance of equipment components. Advancements in technology allowed Beckman Coulter to add a moisture sensor to our HIAC PODS+ Portable Liquid Particle Counters. This application note details the moisture detection application in hydraulic fluid, oil and fuels.
High Throughput Screening {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	For drug discovery and biomarker research, screening campaigns are employed to identify potential new treatments for diseases and to answer questions that remain unknown in the scientific community.
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Beckman Coulter AMPure XP kit is a highly efficient PCR purification system that delivers superior quality DNA with no salt carryover
Method for Determining Cell Type Parameter Adjustment to Match Legacy Vi-CELL XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note assists in matching Vi-CELL BLU cell types to give cell counts and viability levels equivalent to those obtained on existing Vi-CELL XR instruments.
Biomek i-Series1 Automated FormaPure DNA Extraction kit for FFPE Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Extraction of high quality DNA from formalin fixed paraffin embedded (FFPE) tissue is essential for many cancer research applications
Transfection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The introduction of exogenous nucleic acids into cells is an essential means of studying cell biology.
Automated TruSeq RNA Sample Preparation from FFPE Tissue Specimens Utilizing the Biomek FXp Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Bee Na Lee Ph.D., Senior Application Scientist, and Sarah Simons, Advanced Specialist- Field Systems Support, Beckman Coulter Life Sciences \| Michaela Bowden Ph.D., Research Scientist \| Ashley Rossi, Molecular Technician, and Ed Stack Ph.D., Associate Director Center for Molecular Oncologic Pathology, Medical Oncology at DFCI \| Svitlana Tyekucheva, Department of Biostatistics, DFCI Biostatistics & Computational Biology and Massimo Loda MD., Director Center for Molecular Oncologic Pathology, Medical Oncology at DFCI and Department of Pathology at Brigham and Women's Hospital
Illumina TruSeq Nano DNA Library Prep Kit {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Illumina TruSeq Nano DNA Library Prep Kit enables preparation of uniquely indexed DNA libraries for single end and paired end sequencing
Meet the Demand of Illumina’s High-Capacity NGS Platforms with Biomek Automated Sample Preparation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Improve Sample Quality with Standardized Sample Prep \| Quick Implementation with Demonstrated Methods \| Increase Throughput to Maximize Sequencing Runs
Automated Liquid Handling for Cell Staining and Imaging {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski, Staff Applications Scientist \| Li Liu, Sr. Development Scientist \| Amy Yoder, Staff Development Scientist \| Beckman Coulter Life Sciences Indianapolis, IN
Biomek Automated NGS Solutions Accelerate Genomic Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq Exome Library Prep kit is a workflow to generate indexed paired-end libraries and is specially designed to work with Formalin Fixed Paraffin Embedded (FFPE) samples
Automated Size Selection using SPRIselect Beads and the Biomek FX Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Alexandre Bélisle, Vicky Arsenault, Belisle Tir, Alfredo Staffa Sylvie LaBoissière and Alexandre Montpetit - McGill University and Génome Québec Innovation Centre - Montréal, Québec, Canada
Workflow Questions Answered: Challenges with FFPE Tissue Samples {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Jung Doh, Ph.D., Senior Applications Scientist, Beckman Coulter, Inc. \| Published 2017
Evaluation of an Automated Instrument for Viability and Concentration Measurements of Cryopreserved Hematopoietic Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: S. E. SZABO, S. L. MONROE, S. FIORINO, J. BITZAN, K. LOPER \| The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Hospital, Baltimore, Maryland; Beckman Coulter Miami, Florida, USA \| Published 2004
Full Automation of the SISCAPA® Workflow Using a Biomek NXP Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Morty Razavi, Michael Kowalski, Tara Jones-Roe, Christie Hunter, Leigh Anderson, Richard Yip, Matt Pope, Terry Pearson SISCAPA Assay Technologies, Washington DC
Improved Sequencing Performance with Publication-Quality Data {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt CosMCPrep system is a versatile plasmid purification procedure
A Path to the Standardization of Exosome Isolation and NGS Characterization for Complex Disease Studies {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: CHAD SCHWARTZ, PH.D. \| ZACH SMITH, M.S. \| Published October 2015
Highly Efficient MicroRNA and Total RNA Purification from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We describe how the Agencourt SPRI (Solid Phase Reverse Immobilization) magnetic bead based method is superior to the column purification method for purifying miRNA from formalin-fixed paraffin-embedded tissues (FFPE).
Agencourt CleanSEQ System - Dye Terminator Removal {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt CleanSEQ is a SPRID (Solid Phase Reversible Immobilization) paramagnetic bead-based sequencing purification system with a simple three-step protocol
High Throughput TruSeq Stranded mRNA Library Construction on the Biomek FXP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith \| Scott D. Michaels \| Mary Blair
Agencourt RNAdvance Tissue System - Total RNA Extraction from Tissue {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Agencourt RNAdvance Tissue extraction technology, in combination with Beckman Coulter automation, offers researchers a true walk-away solution for consistent recovery of high quality total RNA in a multi-well format
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sample quality is of utmost importance to genomic researchers to better ensure good results when running laborious and expensive processes such as Next Generation Sequencing.
JP SDBS Validation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	JP 16 specifies that when measuring a 500ppb standard of sodium dodecylbenzenesuflonate (SDBS), a TOC analyzer should recover a value of at least 450ppb (90% recovery)
Preparing Reagent Solution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The QbD1200 uses a mixture of phosphoric acid (to aid in removal of CO2 from solution) and ammonium persulfate (to aid in oxidizing organic compounds)
New HIAC PODS+ …Have Internal Pump, Will Travel! {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
Overload Recovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In a typical laboratory environment, a TOC analyzer is used to measure water samples where the TOC value of those samples is unknown
HIAC PODS+ Reporting Standards Synopsis {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
HIAC PODS+ vs. Parker ACM-20 Performance Comparison {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2016
Density Gradient Separation of Gold Nanorods - Using the New Avanti JXN Centrifuge & JS-24.15 Swinging Bucket Rotor {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Gold Nanorods (AuNR) hold great promise for biomedical imaging
HIAC PODS+ Online Mode & Filter Cart Mode {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Joe Dabbs & Bill F. Bars \| Beckman Coulter, Inc., 481 California Ave Grants Pass, OR 97526 USA \| Published 2017
Characterizing RNA Nanoparticles by Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Chad T. Schwartz, Daniel Jasinski, and Peixuan Guo - Beckman Coulter, Inc., Indianapolis, IN \| University of Kentucky, Department of Pharmaceutical Sciences
g-Max: Added Capabilities to Beckman Coulter’s Versatile Ultracentrifuge Line {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Chad Schwartz, Ph.D., Application Scientist I \| Randy Lockner, M.S., Centrifugation Marketing \| Randy Pawlovich, Director of Product Management & Strategy \| Beckman Coulter, Inc., Indianapolis, IN USA
ICH Q2 – the Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Tony Harrison - Compliance and Applications Specialist for Beckman Coulter Life Sciences \| Published 2013
Accurate, Rapid Sizing of C60 Fullerene Particles {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Joshua T. Robinson \| Published 2016
Using k-Factor to Compare Rotor Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The k-factor is a common parameter that describes the efficiency of a centrifuge-rotor system, although temperature is still overlooked
Comparing Data Quality & Optical Resolution of the Next Generation Optima AUC to the Proven ProteomeLab on a Model Protein System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Julia P Luciano-Chadee - Beckman Coulter, Inc., Indianapolis, IN \| Chad Schwartz Ph.D. - Beckman Coulter, Inc., Indianapolis, IN
Use of Beckman Coulter Reagents for Automated, Whole Blood, White Blood Cell Viability Measurements {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Stephen E. Szabo, Ph.D. \| Sarah Monroe, B.S.\| Beckman Coulter, Inc \| Published 2004
A High Recovery and High Purity Data Analysis Strategy for Rare Abnormal Plasma Cell Events using the DuraClone RE PC Tube {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr Agnieszka Blum- Charité Virchow Hospital, Stem Cell Facility, Berlin, Germany \| Dr Michael Kapinsky - Life Science, Beckman Coulter, Marseille, France
Instrument-to-Instrument Variability in the Vi-CELL Automated Viability Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Clarence Lew, Senior Applications Scientist, John A. Gomez, Senior Staff Technical Operations Engineer, Matthew N. Rhyner, Ph.D., Global Marketing Manager-Analytical Products \| Beckman Coulter Life Sciences, Brea, CA USA \| Published 2012
Automated Cord Blood Cell Viability and Concentration Measurements Using the Vi-Cell™ XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Stephen E. Szabo, Ph.D. \| Sarah Monroe, B.S. \| Beckman Coulter, Inc. \| Published 2004
Automated Optimization of Murine Embryonic Stem Cell Differentiation into Cardiomyocytes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski Ph.D., Li Liu Ph.D., Amy Yoder, and Laura Pajak Ph.D. \| Life Science Automation, Beckman Coulter, Inc. Indianapolis, IN \| Published 2011
Automated Sample Preparation Improves Throughput for Flow Cytometric Analysis of Mouse Stem Cell Populations {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: William L. Godfrey, Li Liu, Amy Yoder, and Laura Pajak \| Beckman Coulter, Inc., Miami, FL, USA and Indianapolis, IN, USA
Enhanced Forward Scatter Module+ on the MoFlo™ Astrios: Small Particle Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr. Carley D. Ross, Alan Dean Ph.D., Kerry Weinberg, Robin Morris, Blake McCarty and Nicole Lewis \| Beckman Coulter, Inc., Flow Cytometry Business Center, Fort Collins, CO, USA
MoFlo™ Viability and Function with High-Speed Sorting {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sustained cell sorting at speeds in excess of 50,000 events per second is routine when using the MoFlo high-performance cell sorter
MoFlo™ Stem Cell Sorting {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Purified populations of functional stem cells are of great interest to the biomedical community, both in the understanding of stem cell biology and in clinical transplant settings
MoFlo™ Astrios* Enhanced Forward Scatter+: 0.2 μm to 30 μm Dynamic FSC Detection {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr. Carley D. Ross, Mike Morrell, Robin Morris, Blake McCarty, Alan Dean Ph.D. and Nicole Lewis \| Beckman Coulter, Inc., Flow Cytometry Business Center, Fort Collins, CO, USA
Intelligent Biosafety: MoFlo Astrios Fully Integrated with the Biosafety Cabinet and Aerosol Evacuation System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As with any sorter that requires jet-in-air sorting, aerosols are created both from the sort and waste streams
Rapid and Easy Intracellular + Surface Staining of MOUSE Cells using PerFix-nc {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Dispense 50 μL of cells into empty tube \| Add 5 μL of fixative reagent R1 (low fixation), mix tube \| Incubate 15 min. at room temperature (RT)
High-Throughput Illumina® TruSeq® Nano DNA Library Construction on the Biomek FXP Dual-Arm Multi-96 and Span-8 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Mary Blair
Automated Directional RNASeq Library Preparation using the Biomek FXP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We describe the automation of the New England Biolabs NEBNext (Registered trademark) Ultra Directional RNA Library Prep Kit for Illumina on the Beckman Coulter Biomek FXP automated liquid handler.
Automated Cell Culture: Improving the Consistency of Cell Maintenance {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we illustrate the capabilities for automating a standard workflow for cell line maintenance and demonstrate this process using mouse embryonic stem cells.
Automated Illumina TruSeq DNA PCR-Free Sample Preparation on the Biomek 4000 Laboratory Automated Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: David Horvath, M.S. \| Beckman Coulter, Inc.
Xtra Pure RNA {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt RNAClean XP System, Enzymatic Purification System
AGENCOURT® FormaPure®AGENCOURT® FormaPure® for miRNA and total RNA for miRNA and total RNA {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Beckman Coulter Life Sciences
High Recovery of DNA for Downstream Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Agencourt Genfind v2 system, genomic DNA isolation kit.
Illumina TruSight® HLA Sequencing Panel Automated on the Biomek FXP HLA SP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We describe the automation of the Illumina TruSight® HLA Sequencing Panel on the Beckman Coulter Biomek FXP HLA SP.
Improved Automated Sample Preparation for the Analysis of 25-OH-Vitamin D3 by LC/MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	We demonstrate an improved automated sample preparation for liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis of 25-OH-Vitamin D3.
Automated Sample Preparation for the Monitoring of Ethanol Metabolites in Urine by LC-MS/MS {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we describe an LC-MS/MS method for the analysis of EtG and EtS in urine employing a simple, automated sample preparation methodology using a liquid handling-based automation system.
Real Time Determination Of Cell Volume Changes Of Sperm Used For Cryopreservation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Much of the cellular damage produced by freezing is due to freezing-induced dehydration and thawing-induced rehydration.
Automating 3-D Cell Culture and Screening by Flow Cytometry and High-Content Imaging {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Michael Kowalski, Ph.D. \| Staff applications scientist for Beckman Coulter Life Sciences Indianapolis, IN \| Published 2016
Enzymatic Determination Of Glycerol Contents In Biological Samples Using The Biomek 4000 Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Dr. Jörg Mampel, B.R.A.I.N Aktiengesellschaft, Darmstaedter Str. 34-36, D-64673 Zwingenberg \| Published 2014
High Throughput TruSeq Stranded mRNA Library Construction on the Biomek FXP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Zach Smith and Scott D. Michaels - The Center for Genomics and Bioinformatics - Indiana University \| Mary Blair - Life Science Division Beckman Coulter - Indianapolis, IN \| Published 2013
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Illumina TruSeq DNA PCR-Free Sample Preparation workflow is designed to generate paired-end libraries without the need for PCR amplification
ISO11171-2010 Standard Requirement Changes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Bill F. Bars - Applications Scientist for Beckman Coulter, Inc. in Grants Pass, Oregon, USA
Challenges in Next Gen Sequencing and How to Overcome Them {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The exponential acceleration in our capacity to sequence 3.2 billion human base pairs has transformed our approach to therapeutics, particularly for cancer.
Cell Culture Automating Cell Maintenance {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell culture is an essential workflow that enables studies to be performed in drug discovery and scientific
Cell-Based Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell Culture is an essential workflow that enables studies to be performed in drug discovery and scientific research
Cell Line Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	As biologics research continues to evolve and gain prominence in therapy development for disease states, the need for reliable and robust cell lines will greatly increase
Automated Directional RNASeq Library Preparation using the Biomek FXP Liquid Handler {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Author: Zach Smith, M.S. Beckman Coulter, Inc. \| Published 2014
Fully-Automated Cellular Analysis by Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Direct integration of CytoFLEX Flow Cytometer to Biomek i-Series Automated Workstations enables complete automation of sample processing and data acquisition
Applications of Ultracentrifugation in Purification and Characterization of Biomolecules {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Ultracentrifuges spin samples with centrifugal forces typically spanning 100,000 – 1,000,000 x g. At these high forces, the constituent molecules in the sample separate based on their physical properties (e.g., size, mass, density, anisotropy).
Automated Cord Blood Cell Viability and Concentration Measurements Using the Vi‑CELL XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes a method for cord blood sample preparation and developing a cell type and accurate set of instrument parameters for the Vi-CELL XR.
Cell Counting Performance of Vi–Cell BLU Cell Viability Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	To determine the cell counting performance of the Vi–CELL BLU we adapted the protocol outline by NIST to determine the performance of the new Vi–Cell BLU Cell Viability Analyzer.
HIAC Industrial – Our overview solution for fluid power testing for all applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Compensation Setup For High Content DURAClone Reagents {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	<span>In this DURAClone IM Tube application note, you will how our proprietary dry reagent formulation secures long-term fluorochrome stability while stored at room temperature. </span>
Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube-based DURAClone dry reagent {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Using the 10 color DuraClone IM T Cell Subsets kit plus 3 additional liquid markers, a 13 color tube has been designed for the phenotypic analysis of major T cell sub-populations in the human peripheral blood.
Scalable Plasmid Purification using CosMCPrep {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note demonstrates the flexibility of CosMCPrep, a scalable plasmid purification kit
17-Marker, 18-Color Human Blood Phenotyping Made Easy with Flow Cytometry {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note describes a method for phenotyping human blood using 18 color parameter (multicolor) flow cytometry.
Linearity of BSA Using Absorbance & Interference Optics {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Optima AUC is the latest innovation from Beckman Coulter and offers significant advantages as an analytical ultracentrifugation platform over the predecessor ProteomeLab. Besides the plethora of advancements in user experience on the new platform, the Optima AUC’s optical, thermal, and drive systems have also been upgraded significantly, creating an overall better experience to the end-user in terms of data quality and ease of use.
A Standardized, Automated Approach For Exosome Isolation And Characterization Using Beckman Coulter Instrumentation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Exosomes are small microvesicles, derived from the late endosome, most often described in the literature to be less<br> than 120 nm, released by all cell types, and proven to be involved in cancer metastasis.
Biomek Automated NGS Solutions Accelerate Genomic Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features the Biomek i7 automated genomic workstation and focuses on automated NGS solutions
Efficient kit-free nucleic acid isolation uses a combination of precipitation and centrifugation separation methods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Immunotherapy and gene therapy requires highly purified DNA to deliver a gene editing message, or plasmid, to the host cell. Kit free plasmid miniprep has been traditionally used to deliver high quality and high yield DNA. This method is accomplished by a combination of salts and isopropanol and the efficient precipitation of nucleic acids from solutions via centrifugation. It is high-throughput, cost efficient and it produces higher yields as there is no loss of material due to membrane or filter interaction.
Classifying a Small Cleanroom using the MET ONE HHPC 6+ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This applications note will demonstrate the classification of a small ISO Class 5 Clean room and associated ISO Class 7 gowning room using a MET ONE model HHPC 6+ handheld particle counter. Reference will be made to the ISO 14644-1 clean room classification standard with regards to sample size, locations selected, limits and calculations necessary to determine the compliance status of the clean zone to ISO 14644-1 Class 5.
Counting Efficiency: MET ONE Air Particle Counters and Compliance to ISO-21501 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The one parameter that stands alone as the champion of confusion for defining air particle counter performance is counting efficiency. The purpose of this paper is to present counting efficiency in terms that are easy to understand.
Particle Counting in Mining Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	How Particle Counters can Extend Machine Uptime, Oil Lifetime and Give More Reliability
Critical Particle Size Distribution for Cement using Laser Diffraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Producing high performance cement is directly related to maintaining a very specific Particle Size Distribution. This application note details how Laser Diffraction is quickly becoming the method of choice as it yields a clear and accurate picture of the entire PSD.
Detecting Moisture in Hydraulic Fluid, Oil and Fuels	Monitoring the amount of moisture in petroleum based fluids before 100% saturation occurs will reduce degradation and will increase the life and performance of equipment components. Advancements in technology allowed Beckman Coulter to add a moisture sensor to our HIAC PODS+ Portable Liquid Particle Counters. This application note details the moisture detection application in hydraulic fluid, oil and fuels.
Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Insulin is a well characterized drug used to treat diabetes. A large number of insulin formulations have been investigated in order to develop the optimum time delivery suited to patient needs. In addition, a number of insulin analogs such as Insulin Glargine and LysPro insulin (Humalog) have been developed and marketed as derivatives for immediate use at meal time and a more sustained release.
Flexible ELISA Automation with the Biomek i5 Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features the Biomek i-Series, specifically the Biomek i5 and automating an ELISA assay workflow
Automated Transfection Methods {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features automation of the optimization of transfection conditions through the approach of factorial design
Specification Comparison of Vi–CELL XR and Vi–CELL BLU {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The Vi–CELL BLU leverages the key performance features of the Vi–CELL XR but incorporates many design improvements that our customers have requested over the years. This paper highlights the differences in specifications between the two instruments.
Method for Determining Cell Type Parameter Adjustment to Match Legacy Vi CELL XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note details how you can use the plate loader feature on the Vi-CELL BLU to match cell types to give cell counts and viability levels equivalent to those obtained on existing Vi-CELL XR instruments.
Switching from Oil Testing to Water and back using the HIAC 8011+ and HIAC PODS+ {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Matching Cell Counts between Vi–CELL XR and Vi–CELL BLU {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	<p>This paper demonstrates a series of different cell types run on the Vi-CELL XR and duplicate samples run on the Vi-CELL BLU. The cells were then reanalyzed adjusting the cell type parameters until a new cell type was generated that gave cell concentrations within +/–5%* and viable cell density levels within +/–2.5%* of the Vi-CELL XR. </p> <ol> </ol>
Vaporized Hydrogen Peroxide Decontamination of Vi–CELL BLU Instrument <br> {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Whole instrument decontamination is desirable in many laboratories and necessary in some when using biologically hazardous materials. By necessity such methods are chemically aggressive in nature and can cause serious damage to electronic and optical systems. The Vi–CELL BLU Cell Viability Analyzer was tested for VHP resistance. This paper outlines the method and results.
The New Avanti J-15 Centrifuge Time Saving Deceleration Profile Improves Workflow Efficiency {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note by Beckman Coulter Life Sciences demonstrates the the new Avanti J-15 centrifuge deceleration profile
Vi–CELL BLU FAST Mode Option<br> <br> {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper explains the optional FAST Mode of operation in the Vi-CELL BLU cell viability analyzer which can be used in either plate or carousel configuration to significantly reduce sample to sample operation times. <br>
Changes to GMP Force Cleanroom Re-Classifications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper explains the new rules of ISO 14644-1:2015, which has substantial revisions impacting both the way the cleanrooms are classified and the performance requirements of the air particle counting instruments used to carry out the classification.
Cleanroom Routine Environmental Monitoring – FDA Guidance on 21 CFR Part 11 Data Integrity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper examines the risk of error with SOPs being implemented manually. Technology exists and is commercial available to mitigate this problem and make programs more robust, reducing the impact on data integrity and also supporting the industry's move towards environmental monitoring by production staff in the cleanroom.
Control Standards and Method Recommendations for the LS 13 320 XR {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Conducting the ISO 14644-3 Cleanroom Recovery Test with the MET ONE 3400 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note reviews the test method and provides guidance on selecting both the “Challenge” and the “Target Cleanliness Limit”. Attention is given to the requirement that the test concentration should not be so high that “residue contamination” becomes a risk.
Quality Control Electronic Records for 21 CFR part 11 Compliance {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper describes how Quality Control instruments can be optimised for pharmaceutical use, helping to improve the integrity of the data in the final electronic record.
Importance of TOC measurement in WFI in light of European Pharmacopoeia change {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper discusses the increased focus on Total Organic Carbon (TOC) and conductivity measurement, especially for companies seeking to use RO water treatment plants to create WFI, and provides best-practice advice for calibration.
Automating Biopharma Quality Control to Reduce Costs and Improve Data Integrity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper takes a look at four common quality control procedures and how automation can help improve data integrity by reducing opportunities for human error, whilst also saving time and reducing operating costs. The four quality control procedures discussed in this paper form a common thread in the biopharmaceutical manufacturing industry and are water quality testing, cleanroom routine environmental monitoring, biological cell counting and viability testing and final injectable drug particulate testing.
21 CFR Part 11 Data Integrity for On-line WFI Instruments {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note takes a look at how quality-critical on-line Total Organic Carbon (TOC) and conductivity instrumentation for WFI (water for injection) quality control can be configured to help companies comply with the FDA’s expectations on data integrity, including the use of Microsoft Active Directory and PDF data export.
Vi-CELL BLU Regulatory Compliance - 21 CFR Part 11 {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	The following information describes the relevant portions of the 21 CFR Part 11 regulations and their implementation using the Vi-CELL BLU control software.
European Pharmacopoeia EP 2.2.44 and Total Organic Carbon {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper discusses the background behind the European Pharmacopoeia’s focus on complete oxidation of TOC in the light of the increased importance of on-line Total Organic Carbon (TOC) and conductivity measurement.
Achieving Compliant Batch Release – Sterile Parenteral Quality Control {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper describes common QC instrumentation compliance elements and gives examples of best practice for instruments used for compliant QC batch release.
Evaluation of Instrument to Instrument Performance of the Vi-CELL BLU Cell Viability Analyzer {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Viral Nucleic Acid Extraction {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we report the ability to extract DNA from samples containing HBV
Modern Trends in Non‐Viable Particle Monitoring during Aseptic Processing {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper describes changes and improvements to non‐viable particle monitoring (NVP), sometimes referred to as total particulate monitoring, which is a regulatory requirement during aseptic processing. Aseptic processing is becoming more automated and increasingly important to future products of the biopharmaceutical industry. In the same manner, NVP monitoring is also becoming more automated and increasingly important for contamination risk management during aseptic processing.
Avoid the Pitfalls When Automating Cell Viability Counting for Biopharmaceutical Quality Control {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this paper we will show one example of how identifying viable cells by minimum diameter alone can introduce variability of circa 33% in the reported cell concentration. This paper describes how to avoid these common pitfalls when a user is adopting a new automated method of cell counting.
Considerations of Cell Counting Analysis when using Different Types of Cells {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper reviews several different cell types and cell preparation approaches using standard cells, such as, CHO cells, EL4 cells, SF9 cells, and HELA cell cultures.
Leveraging the Vi-CELL MetaFLEX for Monitoring Cell Metabolic Activity {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note presents how our media analyzer Vi-CELL MetaFLEX was tested and the potential benefits for accelerating metabolic rewiring research.
Full Automation of the SISCAPA® Workflow using a Biomek NX<sup>P</sup> Laboratory Automation Workstation {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features the Biomek NX<sup>P</sup> Automated Workstation and the full automation of the SISCAPA® workflow
Automating the Cell Line Development Workflow {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features the Biomek i-Series Automated Workstation - a complete walk-away solution for your cell line development workflow
Automating Cell-Based Processes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note focuses on increasing needs to produce cell lines and minimize the burden of upfront prep and cell culture processing
Automating Bradford Assays {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features Biomek i-Series and supports consistency of automated standard dilution and Bradford assay preparation
Cell Line Development – Selection and Enrichment {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Cell line development is a highly involved and inefficient process. We discuss automation for increased production in this application note
Cell Line Development – Hit Picking {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note we discuss how hit picking was automated as part of a cell line development workflow
The new Avanti J-15 Centrifuge Improves Sample Protection and Maximizes Sample Recovery {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note by Beckman Coulter Life Sciences demonstrates the improved sample protection and recovery
Cell Line Development – Limiting Dilution {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note highlights the automation of limiting dilution on a Biomek i7 liquid handling workstation
Multi-Wavelength Analytical Ultracentrifugation of Human Serum Albumin complexed with Porphyrin {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	<p style="margin-right: 0px; margin-bottom: 0px; margin-left: 0px;">Analytical Ultracentrifugation (AUC) is the method of choice to characterize colloidal systems under physiological</p> <p style="margin: 0px;">solution conditions. Recently, a novel multi-wavelength (MWL) detector on Beckman Coulter’s Optima AUC has</p> <p style="margin: 0px;">been developed, which for the first time, couples the hydrodynamic separation of colloidal mixtures to spectral</p> <p style="margin-top: 0px; margin-right: 0px; margin-left: 0px;">deconvolution of interacting and non-interacting solutes present in a mixture.</p>
Comparing Data Quality & Optical Resolution of the Next Generation Optima AUC to the Proven ProteomeLab on a Model Protein System {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	AUC provides a matrix-free environment that allows the near-native state characterization of a wide range of molecules. Our recently launched Optima AUC is the only commercially available instrument equipped with a state of the art optical system.
Automating Cell Line Development {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features automating cell line development with a Biomek i7 liquid handling workstation and integrated devices in order to overcome challenges for this workflow
Optimizing Workflow Efficiency of Cleanroom Routine Environmental Monitoring {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This paper examines a thoughtfully designed and well-executed environmental monitoring program that tests the controls put in place to maintain the cleanroom to the required Good Manufacturing Practices standards (e.g., CGMP, EU-GMP, and PIC/S).
Biomek Automated Genomic Sample Prep Accelerates Research {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	This application note features Biomek i-Series automated Illumina TruSeq DNA PCR-Free with the spotlight on the Biomek i5 Span-8 Genomic Workstation
Analyzing Mussel/Mollusk Propagation using the Multisizer 4e Coulter Counter {442DF88C-CA7F-4B78-AF75-9D2B985534C0}
Temperature dependence of hydrodynamic radius of an intrinsically disordered protein measured in the Optima AUC analytical ultracentrifuge. {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Analytical ultracentrifugation experiments over a wide range of temperatures were conducted to measure the hydrodynamic radius of an intrinsically disordered protein. Taking advantage of the highly stable temperature control offered through the latest shroud modification available in the new Optima AUC, replicate experiments demonstrated highly reproducible hydrodynamic parameters that suggest slight changes in the hydrodynamic radius of an intrinsically disordered protein, and impressive performance in both temperature control and data quality from the Optima AUC.

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