How can I transfer an existing centrifugation protocol to a new rotor, bottle, or tube?
Protocol transfer is a common occurrence when scaling up a process to higher volume or improving efficiency by increasing speed. Though the process of going through a series of calculations to transfer an existing protocol can be a daunting and time-consuming task, Beckman Coulter offers convenient software tools and online solutions to facilitate seamless protocol transfer.
Simplify Protocol Transfer
Speak the Same Language – RPM vs. RCF
Depending on the source, a centrifugation protocol may be described in units of RPM (revolutions per minute) or RCF (relative centrifugal field) – RCF is also known as g-force (x g) and is typically reported for the maximum radius (rmax). RCF and RPM can be interconverted using the rotor radius, though transferring a protocol to a new tube, bottle, or rotor requires additional information on the existing and new setups.
Equate g-Force with Performance – k-Factor
Switching from one rotor or tube to another often changes the rmin and rmax, and the maximum g-force does not capture efficiency. A metric called the k-factor considers pathlength and RCF to represent pelleting efficiency. The k-factor also accounts for run time and offers simple protocol conversions when using different rotors, tubes or bottles, adapters, and fill volumes.
Simplify Protocol Transfer – Available Tools
Properly transferring a centrifugation protocol requires more than simply matching RPM or RCF values, and tracking down rotor and tube dimensions can be a tedious process. Optima XPN ultracentrifuges include eXpert simulation software, including a streamlined tool for switching to new rotors or tubes. Additionally, the online rotor calculator offers pre-loaded specs to simplify protocol transfer.
Moving a centrifugation protocol to a new setup can be a chore to calculate by hand. Beckman Coulter offers tools to save you time and simplify protocol transfer. Click here to check out the rotor calculator tool, or visit beckman.com/spINSIGHTS to explore other topics.