ClearLLab LS Lymphoid Screen Reagent Sample Data

Normal Bone Marrow

This CD45 x Side Scatter dot plot demonstrates typical components of a normal bone marrow, including granulocytes (blue), monocytes (red), lymphocytes (green), myeloblasts (pink) and B-lymphoid progenitors aka “hematogones” (orange).

This CD19 x CD10 dot plot demonstrates characteristic CD19/CD10 co-expression by hematogones (orange). CD19-positive B lymphocytes (green) and CD10-positive granulocytes (blue) are also noted.

This CD34 x CD19 dot plot demonstrates characteristic CD34 expression by a subset of hematogones (orange) as well as by myeloblasts (pink). CD19-positive B lymphocytes (green) are also noted.

Lymph Node with Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma

This CD45 x Side Scatter dot plot demonstrates both normal T lymphocytes (green) and a large population of cells displaying slightly lower CD45 expression (red).

This CD5 x CD19 dot plot demonstrates both normal CD5-positive T lymphocytes (green) and CD5/CD19 co-expression characteristic of CLL/SLL. Note that this immunophenotype is not specific for CLL/SLL, and additional testing may be warranted to further characterize this process.

Bone Marrow with B Lymphoblastic Leukemia/Lymphoma

This CD45 x Side Scatter dot plot demonstrates both normal T lymphocytes (green) and a large population of cells displaying lower CD45 expression (red).

This CD34 x CD19 dot plot demonstrates CD34/CD19 co-expression characteristic of B lymphoblastic leukemia/lymphoma (red).

This CD5 x CD10 dot plot demonstrates CD10 expression by the B lymphoblastic leukemia/lymphoma (red) and CD5 expression by the normal T lymphocytes (green).

Bone Marrow with Acute Myeloid Leukemia

This CD45 x Side Scatter dot plot demonstrates typical components of a normal bone marrow, including granulocytes (blue) and lymphocytes (green), as well as a large population of cells displaying lower CD45 expression (pink).

This CD34 x Side Scatter dot plot demonstrates CD34 expression by the low density CD45 population (pink). Neither the lymphocytes (green) nor the granulocytes (blue) display CD34 expression.

This CD3 x CD19 dot plot demonstrates that the normal lymphocytes (green) are split into CD19-positive B-cells and CD3-positive T-cells. The granulocytes (blue) are negative for both CD19 and CD3, consistent with their myeloid origin. The CD34-positive cells (pink) are also negative for both CD19 and CD3, as well as for CD2, CD5, and CD7 (data not shown), suggesting that they may represent acute myeloid leukemia. Additional testing is warranted to further characterize this process.