Células-tronco

Ferramentas para melhorar o fluxo de trabalho

A biologia de células tronco emergiu como um dos campos mais significativos na ciência contemporânea apresentando o potencial de uma fonte renovável de células de reposição e tecidos para tratar uma ampla gama de doenças

A Beckman Coulter oferece instrumentação, bem como uma variedade de reagentes com foco em células-tronco para citometria de fluxo, triagem celular, western blotting, ELISAs ( ensaio de imunoabsorção enzimática), diferenciação celular e muito mais.

Videos

Stem Cell Resources

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Title Description
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek® NXP Laboratory Automation Workstation
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To understand the changes that occur during differentiation of murine embryonic stem cells (mESCs)
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek® NXP Laboratory Automation Workstation
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Authors: Amy Yoder, Michael Kowalski Ph.D., Li Liu Ph.D., Laura Pajak Ph.D. | Life Science Automation, Beckman Coulter Inc, Indianapolis, IN
Identifying Brain Tumor Initiating Cells Using Flow Cytometry Application Note
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Tumors represent a heterogenous population. Enumerating cells with stem cell like properties via co-expression of markers provides insight into the evolution of the tumor over time and throughout treatment. Detailed procedure for processing brain tissue for culture and flow cytometric analysis is provided.
Automated Optimization of Murine Embryonic Stem Cell Differentiation into Cardiomyocytes
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Authors: Michael Kowalski Ph.D., Li Liu Ph.D., Amy Yoder, and Laura Pajak Ph.D. | Life Science Automation, Beckman Coulter, Inc. Indianapolis, IN | Published 2011
Automated Sample Preparation Improves Throughput for Flow Cytometric Analysis of Mouse Stem Cell Populations
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Authors: William L. Godfrey, Li Liu, Amy Yoder, and Laura Pajak | Beckman Coulter, Inc., Miami, FL, USA and Indianapolis, IN, USA
MoFlo™ Viability and Function with High-Speed Sorting
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Sustained cell sorting at speeds in excess of 50,000 events per second is routine when using the MoFlo high-performance cell sorter
MoFlo™ Stem Cell Sorting
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Purified populations of functional stem cells are of great interest to the biomedical community, both in the understanding of stem cell biology and in clinical transplant settings
Measuring Proliferation in Leukemia Cell Lines Using CFSE Application Note
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Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye which is cell permeable. It binds covalently to intracellular proteins. Fluorescence decreases over time as the dye is divided between daughter cells at each division. In this application note a detailed protocol for using this assay to assess cytotoxic effects of a pharmacologic agent is described.
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek NXP
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Authors: Amy Yoder, Michael Kowalski Ph.D., Li Liu Ph.D., Laura Pajak Ph.D. | Life Science Automation, Beckman Coulter Inc., Indianapolis, IN | Published 2011

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