Beckman.com is conducting maintenance Saturday, September 19th and Sunday, September 20th. Parts of the site may have limited accessibility throughout the weekend. During this time, orders can still be placed by calling Client Services. We look forward to providing an improved customer experience. Beckman.com will return Monday, September 21st.
What medium should I use when isolating exosomes using ultracentrifugation and a density gradient?
For best results, most researchers use sucrose or another metabolically inert medium such as iodixanol, which is non-ionic and non-toxic to cells. Unlike sucrose, iodixanol is less viscous, and is capable of forming iso-osmotic solutions at all densities, thereby better preserving the size of the vesicles in the gradient.1 This medium is currently available under the brand name OptiPrep™, a sterile, endotoxin-tested solution of 60% iodixanol in water with a density of 1.32 g/mL. The high density of OptiPrep™ facilitates the fractionation of cells by flotation from a dense load zone through either a continuous or discontinuous gradient, or through a simple density barrier.
 Dettenhofer M, Yu XF. Highly purified human immunodeficiency virus type 1 reveals a virtual absence of Vif in virions. J Virol. 73; 1460-7: (1999).
Oops. The page you wanted could not be found, so we brought you to something similar. If you still can't find what you want, try searching our site instead.