AMPure XP Performance and Data
AMPure XP Manual or Automated Purification & Clean-up
Nucleic acid purification and clean-up are mandatory for genomic applications including sequencing, qPCR/ddPCR/PCR, microarrays and other enzymatic reactions. Maximizing recovery, consistency, and speed to facilitate the entire NGS workflow, Agencourt AMPure XP is optimized to meet the stringent needs of today’s genomic applications and to minimize the risk of losing important genetic information.
- High recovery of amplicons, greater than 100 bp
- Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
- Predictable and consistent size selection
NGS Experts Choose AMPure XP
Only AMPure XP provides predictable performance while maintaining efficient recovery proven by the relative difference between the reference and 1.8x ratio in the traces below.
Agilent TapeStation traces of sheared gDNA purified using Ampure XP, Reagent “O”, and Reagent “K”. Clean-ups were performed using 1.8x (red), 0.9x (blue), 0.7x (green), and 0.6x (aqua) reagent to sample ratios as shown. Traces show relative differences in peak height and recovery efficiency. Note the most common ratio used for NGS library clean up is 1.8x.
Visual Workflow
Automation vs. Manual Timing
AMPure XP is a flexible reagent suitable for manual or automated workflows. Below is estimated hands-on time and total time, in minutes, for AMPure XP.
- Scalable based on throughput
- Quick transition with ready-to-implement methods
- Knowledgeable support for reagents, automation and methods all from a single vendor
| AMPure XP | Manual | Automated | ||
|---|---|---|---|---|
| Batch Size | 48 | Hands-on Time | 25 | 5 |
| Total Time | 25 | 22 | ||
| 96 | Hands-on Time | 30 | 5 | |
| Total Time | 30 | 22 | ||
| 4 x 96 | Hands-on Time | NR | 10 | |
| Total Time | NR | 45 | ||
AMPure XP is available in three kit sizes based on your throughput needs.
| PART # | NAME | PREPS |
|---|---|---|
| A63880 | Agencourt AMPure XP, 5 mL | 5 mL |
| A63881 | Agencourt AMPure XP, 60 mL | 60 mL |
| A63882 | Agencourt AMPure XP, 450 mL | 450 mL |
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